
Because the conjugation of dye to proteins in. This enables visualization of both the protein marker and sample proteins on the same western blot without additional reagents. Use the CLEARLY Stained Protein Ladder to easily monitor the efficiency of protein transfer to a Western blot.
Constituents: 0.44% Tris citrate/phosphate, 0.02% Urea, 2% Sodium lauryl sulfate, 0. mPAGE Western Protein Standards consist of seven recombinant proteins at 20 kDa, 30 kDa, 40 kDa, 50 kDa, 60 kDa, 80 kDa and 120 kDa with an IgG binding site that binds to most antibodies. Recommended loading: ~2 - 3 µl fro western blots This prestained protein MW marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. Whatever your needs for protein electrophoresis and western blotting, there is at least one protein ladder that meets all your requirements. For western blotting, use any of the SDS-PAGE ladders or those optimized for chemiluminescence detection. Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands. Ladders for IEF and 2-D SDS-PAGE provide reproducible calibration for pI estimation. Ready-to-use: Supplied in a loading buffer for direct loading on gels. Note: Protein Marker / Protein Standard / SDS-PAGE / Western Blot / 9 bands / SDS-polyacrylamide gel electrophoresis. Convenient and ready-to-use CLEARLY Protein Ladder sets are mixtures of 11 highly purified recombinant proteins. Do not heat, dilute, add reducing agent before loading. The ladder is supplied in gel loading buffer and is ready to use. ladder contains an integral Strep-tag II sequence which can be detected directly on Western blots using a Strep-Tactin-AP conjugate or an antibody.
The ladder is supplied in gel loading buffer and is ready to use.
WESTERN BLOT LADDER VERIFICATION
This prestained protein ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. This prestained protein ladder is designed for monitoring protein separated during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins.This product was previously called Prism Ultra Protein Ladder (10 - 180 kDa).